Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Fisher Scientific PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™
Commonly used buffer
Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled.
Invitrogen™ Tris (1 M), pH 8.0, RNase-free
Invitrogen Molecular biology grade, 1M Tris, pH 8.0 solution is supplied in one bottle. The buffer is certified RNase-free, economical, and ready-to-use.
Fisher Scientific TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
CAS: 10043-35-3 Synonym: TBE
Invitrogen™ 20X Bolt™ MES SDS Running Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
Thermo Scientific™ Pierce™ Concentrated Buffer Stocks (10X and 20X)
Save time using Pierce Concentrated Buffer stock solutions (10X and 20X) with simple dilutions to expedite your experiment without sacrificing quality or consistency.
Fisher Scientific TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™
Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT
TAE Buffer, Tris-Acetate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents
Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)
Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels
Thermo Scientific™ Dry-blend Buffered Packs
Our quality control ensures that every pack of dry-blend buffers yields consistent solutions without weighing or pH adjustment. Simply empty contents of one foil envelope pack into a beaker, add appropriate solvents, and stir to dissolve.
Corning™ Molecular Biology Reagents
Used in molecular biology, nucleic acid purification applications
Invitrogen™ 20X Bolt™ MOPS SDS Running Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
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Gibco™ CTS™ DPBS, without calcium chloride, without magnesium chloride
Gibco Cell Therapy Systems (CTS) DPBS is the classic Dulbecco's phosphate-buffered saline. This particular formulation lacks calcium, magnesium, and phenol red.
Fisher Chemical Tris Hydrochloride, 1M Solution (pH 7.0/Mol. Biol.), Fisher BioReagents™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris-HCl PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O