Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Gibco™ ACK Lysing Buffer
Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow
Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled.
Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled
Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)
Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels
Invitrogen™ 20X Bolt™ MES SDS Running Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
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Gibco™ CTS™ DPBS, without calcium chloride, without magnesium chloride
Gibco Cell Therapy Systems (CTS) DPBS is the classic Dulbecco's phosphate-buffered saline. This particular formulation lacks calcium, magnesium, and phenol red.
Invitrogen™ Tris (1 M), pH 8.0, RNase-free
Invitrogen Molecular biology grade, 1M Tris, pH 8.0 solution is supplied in one bottle. The buffer is certified RNase-free, economical, and ready-to-use.
Invitrogen™ TE, pH 8.0, RNase-free
Invitrogen molecular biology grade, TE, pH 8.0 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use.
Invitrogen™ Tris (1 M), pH 7.0, RNase-free
Invitrogen Molecular biology grade, 1M Tris, pH 7.0 solution is supplied in one bottle. The buffer is certified RNase-free, economical, and ready-to-use.
Invitrogen™ 20X Bolt™ MOPS SDS Running Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 10X), pH 7.4
Non-toxic to most cells and is used in biological research.
Invitrogen™ Novex™ Tricine SDS Running Buffer (10X)
Novex Tricine SDS Running Buffer (10X) is formulated for separation of proteins in their denatured state on tricine gels.
TAE Buffer, Tris-Acetate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents
